chromatin decross (New England Biolabs)
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New England Biolabs
chromatin decross
Chromatin Decross, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chromatin+decross/Proteinase+K/pmc06434407-99-60-73
Average 99 stars, based on 2790 article reviews
Chromatin Decross, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chromatin+decross/Proteinase+K/pmc06434407-99-60-73
Average 99 stars, based on 2790 article reviews
chromatin decross - by Bioz Stars,
2026-10
99/100 stars
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Related Articles
Ligation:Article Title: Single Chromosome Aneuploidy Induces Genome-Wide Perturbation of Nuclear Organization and Gene Expression Article Snippet: Chromatin end overhangs were filled in and marked with biotin-14-dATP (Thermo Fisher Scientific) by adding the following components to the reaction: 37.5μl of 0 .4mM biotin-14-dATP (Life Technologies), 1 .5μl of 10 mM dCTP, 1 .5μl of 10 mM dGTP, 1 .5μl of 10 mM dTTP, and 8 μl of 5 U/μl DNA Polymerase I, Large (Klenow) Fragment (NEB). .. The marked chromatin ends were ligated by adding 900 μl of ligation master mix consisting of 663 μl of water, 120 μl of 10X NEB T4 DNA ligase buffer (NEB), 100 μl of 10% Triton X-100, 12 μl of 10 mg/ml BSA, 5 μl of 400 U/ μl T4 DNA Ligase (NEB), and incubated at room temperature for 4 h. Incubation:Article Title: Single Chromosome Aneuploidy Induces Genome-Wide Perturbation of Nuclear Organization and Gene Expression Article Snippet: Chromatin end overhangs were filled in and marked with biotin-14-dATP (Thermo Fisher Scientific) by adding the following components to the reaction: 37.5μl of 0 .4mM biotin-14-dATP (Life Technologies), 1 .5μl of 10 mM dCTP, 1 .5μl of 10 mM dGTP, 1 .5μl of 10 mM dTTP, and 8 μl of 5 U/μl DNA Polymerase I, Large (Klenow) Fragment (NEB). .. The marked chromatin ends were ligated by adding 900 μl of ligation master mix consisting of 663 μl of water, 120 μl of 10X NEB T4 DNA ligase buffer (NEB), 100 μl of 10% Triton X-100, 12 μl of 10 mg/ml BSA, 5 μl of 400 U/ μl T4 DNA Ligase (NEB), and incubated at room temperature for 4 h. |